Effect of graded doses of Caesalpinia bonducella seed extract on ovary and uterus in albino rats. Article (PDF Available) in Journal of basic and. Objective: The study is aimed to evaluate anti-inflammatory activity of Caesalpinia bonducella Fleming (Caesalpiniaceae) flower extract (CBFE) and to study its. Fitoterapia. Dec;77() Epub Jul 6. Antidiabetic activity of Caesalpinia bonducella seed extracts in rats. Kannur DM(1), Hukkeri VI, Akki KS.
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The study is aimed to evaluate anti-inflammatory activity of Caesalpinia bonducella Fleming Caesalpiniaceae flower caezalpinia CBFE and to study its effect on radiographic outcome in adjuvant induced arthritis and authentication by high performance thin layer chromatography HPTLC chemical fingerprinting. Effect on radiographic outcome was tested in adjuvant-induced arthritis. The extract was found to possess significant anti-inflammatory activity.
CBFE treatment improved the bony architecture in adjuvant-induced arthritis bonduceloa rats. Caesalpinia bonducella Fleming Caesalpiniaceae is a shrub found in coastal areas of South Asian countries. Various parts are used for different purposes in ethno pharmacology. Infusions of flowers were prescribed in bronchitis, as tonic and emmenagogue and found to possess anticancer properties.
Chemical fingerprinting will serve as an effective tool in authentication and quality control of herbs. Chromatography can readily ascertain the presence of the essential chemical constituents of a medicinal plant and detection of their presence in a drug preparation. High performance thin layer chromatography HPTLC is being used for fingerprint profiling of medicinal plant extracts since long. Bonducrlla fingerprint profile has been proved to be an effective tool in differentiating closely acesalpinia species and detecting adulteration and substitution in raw drugs of Indian systems of medicine.
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Hence, this study was carried out to investigate anti-inflammatory activity of CBFE and to study its effect on radiographic outcome in adjuvant induced arthritis and its authentication by HPTLC chemical finger printing.
The voucher specimen ID: The extract was concentrated under reduced pressure and made free of solvent to obtain a semisolid mass yield 6. In the preliminary phytochemical screening, CBFE was found to have flavonoids, terpene, tannin, and chlorophyll.
The extract was concentrated to 10 ml solution under boneucella pressure.
Antidiabetic activity of Caesalpinia bonducella seed extracts in rats.
The plate was developed in a solvent system of toluene and ethyl acetate in the proportion of 5 and 1. R f values of the spots were recorded. All chemicals were of analytical grade and most of them were procured from Sigma Chemical Co.
Wistar nonducella — g and mice of either sex were bondycella from the Laboratory Animal Resource Section of the Institute and acclimatized to the laboratory conditions at least 1 week before experimentation. The animals were kept under standard environmental conditions and maintained caeszlpinia a standard rodent diet provided by Feed Technology Unit of the Institute and water was provided ad libitum.
The experimental procedures were duly approved EC No. The extract was administered orally to four groups of four mice each 3, 4.
Adult male wistar rats weighing — g were randomly divided into five groups containing six animals each. Animals were fasted for 16 h but allowed fresh water ad libitum. The paw volume 0 h was measured using plethysmometer Ugo Basile, Italy. Group I which served as control received vehicle. CBFE and reference drug was administered orally 1 h before injection of phlogistic agent to the hind paw. Carrageenan was injected 0.
The volume of injected paw of each rat was measured 3 and 5 h later. The edema volume ml was calculated by subtracting the 0 h reading from 3 to 5 h reading. From the mean edema volume, the percentage of inhibition of edema was calculated between treated and control groups.
The effect of the extract boneucella studied in proliferative phase of inflammation induced by cotton pellet in wistar rats. From the day of cotton pellet insertion, for 7 consecutive days the oral administration of the drugs was done. Percent inhibition was compared with the untreated control group. Group I served as control group which received vehicle. The difference from the normal paw volume was taken as the edema volume at corresponding time intervals and the percent inhibition of edema volume was calculated at different time intervals between the treated and control groups.
This test is based on the procedure[ 14 ] in which the rats were randomly divided into four groups of eight animals each. Group I served as control and received the vehicle.
On day 1, 6 h after dosing, half of the group 4 animals caesa,pinia sacrificed, and the stomach was removed, cut along the greater curvature. Contents were washed and mucosal surface checked for presence of ulcers. Same procedure was adopted on day 4 to study the chronic ulcerogenic activity with the remaining 4 animals in all groups. Ulcerogenic score following caedalpinia 5 point scale was employed. In this experiment, young female rats weighing 90— g were selected and divided into six animals each.
The grinding surface of the pestle was moistened with water and Mycobacterium was ground to fine powder. One-third of the mineral oil caesalpinoa added to the mortar and the mixture was ground to create fine suspension.
This procedure was repeated until all the mineral oil has been added to make the mixture into fine suspension. This mixture was prepared fresh. On day 1, the freshly prepared adjuvant was injected 0. Pressure was maintained at the site of injection to prevent leakage.
Group I served as a vehicle-treated control. Group V was given indomethacin 0. Animals were X-rayed before inducing arthritis to confirm the casealpinia of boney lesions. Anesthetized rats were placed on a radiographic box at a distance of cm from the X-ray source. Radiographic analysis of normal and arthritic hind paws was performed using X-ray machine with a 48 k Vp exposure for 0.
A blind and independent assessment of the radiological score was performed by two observers.
The following radiological criteria were considered: Score 1, tissue swelling and edema; score 2, joint erosion; score 3, bone erosion and osteophyte formation. The results of orally administered CBFE on cotton pellet granuloma test in rats are summarized in Table 2. Table 4 shows the ulcerogenic score of CBFE for acute as well as chronic administration. CBFE did not produce any ulcer in both the study whereas the reference drug indomethocin which produced strong ulcers both in acute as well as chronic administration with the ulcerogenic score of four.
Figure 4 depicts the radiographic pictures of normal, arthritic, CBFE at 30 mg, mg, mg and reference drug indomethacine treated arthritic groups. Representative radiographic picture of normal, arthritic, Caesalpinia bonducella Fleming Caesalpiniaceae flower extract at 30 mg, mg, mg and reference drug indomethacine treated groups.
High performance thin layer chromatography finger print profile with its Rf value of Caesalpinia bonducella Fleming Caesalpiniaceae flower extract under nm.
Table 5 shows the radiographic score of the study. In negative arthritic animals, the mediolateral radiograph of pelvic limb taken before induction of arthritis showed long bones having normal radiographic density and appearance, sharp articular surfaces and well defined joint space. The metatarsus was relatively less dense. The tarsal joints were well formed with normal tarsus.
Positive arthritic animals showed soft tissue swelling with lysis of epiphyseal end of the tibia. There was fluffy appearance of articular ends of tibia and femur as well. The changes were more pronounced in tarsal than stifle joint.
Soft tissue swelling and osteolysis of articular surfaces of long bones were comparable to that of positive arthritic animals. Soft tissue swelling seen in animals treated with mg CBFE was more or less comparable to those of arthritic animals.
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The integrity of articular surfaces on long bones was, however, maintained. The articular surfaces of long bones particularly those forming stifle joint appeared fluffy. The bones which formed the tarsal joint, however, had the normal radiological appearance. In indomethacin treated animals, mild degree of soft tissue swelling was seen around the articular joint.
The integrity of bone and joints was, however, maintained. In radiographic score assessment significant reduction in radiographic score observed both in cesalpinia CBFE treatment and indomethacin treatment as well. High performance thin layer chromatography finger print profile along with its Rf ccaesalpinia of Caesalpinia bonducella Fleming Caesalpiniaceae flower extract after derivatisation with vanillin-sulphuric acid reagent.
In this study, acute inflammation was induced by injecting 0. The development of edema in the paw of rat after injection of carrageenan is a biphasic event.
We observed that the effect was dose-dependent at 30 and mg of CBFE. Hence, it can be assumed that at these dose levels CBFE is more effective. To assess the efficacy of CBFE against chronic inflammation, cotton pellet granuloma test was employed.
This model is an indicator of proliferative phase of inflammation. Inflammation involves proliferation of macrophages, neutrophils, fibroblasts, and multiplication of small blood vessels, which are the basic source of forming a highly vascularized reddish mass, termed granulation tissue. Along with flavonoids and terpinoids, other phytochemical principles such as tannins may also contribute to the anti-inflammatory effect of CBFE.
Potent anti-inflammatory activity of C. They sensitize the nerve ending for pain sensation and enhance the inflammatory mediators to be released at the site of insult. Most of the anti-inflammatory drugs possessing varying levels on inhibition at autocoid level potentiate the anti-inflammatory effect.
Prostaglandins produced by inflamed tissue sensitize nociceptors to inflammatory mediators such as bradykinin and 5-HT. In our laboratory conditions, young female rats weighing 90— g alone developed arthritis effectively. These animals developed chronic swelling in multiple joints, with influx of inflammatory cells, erosion of joint cartilage and bone destruction. These inflammatory changes ultimately result in the complete destruction of joint integrity and function of the affected joints.
Anti-inflammatory drugs like cyclooxygenase COX inhibitors, such as indomethacin, which has been useful for the treatment in human diseases, ameliorates the joint inflammation in this rat model.
Radiographs provide a measure of damage that occurs in patients with arthritis. Macrophages are the initiators of the pathogenic cascade in rheumatic arthritis pathology. When macrophages are activated in the synovial tissue, they are involved in the activation of inflammatory cells, cell contact, over expression of major histocompatibility complex class II molecules and cytokine production.